An improved method for acute isolation of neurons from the hippocampus of adult rats suitable for patch-clamping study / 生理学报
Acta Physiologica Sinica
;
(6): 112-117, 2004.
Article
Dans Anglais
| WPRIM
| ID: wpr-352805
ABSTRACT
An improved method is described for fast and reliable isolation of neurons from hippocampus of adult rats by a combination of mechanical and enzymatic means. The procedure allows the isolation of neurons from 500-600-d-old rats (over 300 g), preserving the proximal dendritic structure without impairing the electrical characteristics of the cells. Morphologically distinct neurons can be recognized. Using cell-attached, inside-out and whole-cell configurations of patch clamp technique, it was shown that the enzymatically isolated neurons in hippocampus from rats weighing more than 300 g exhibited voltage-gated calcium, sodium and potassium currents, outwardly rectifying chloride channel and large conductance Ca(2+)-activated potassium channel currents. Approximately, 95% of healthy cells allowed the formation of giga-ohm seals.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Ouverture et fermeture des portes des canaux ioniques
/
Séparation cellulaire
/
Rat Wistar
/
Techniques de patch-clamp
/
Biologie cellulaire
/
Canaux potassiques calcium-dépendants
/
Hippocampe
/
Méthodes
/
Neurones
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Acta Physiologica Sinica
Année:
2004
Type:
Article
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