Effect of fluid flow-induced shear stress on osteoclast formation induced by osteocyte / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 207-211, 2012.
Article
Dans Anglais
| WPRIM
| ID: wpr-352927
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of osteocyte in bone remodeling due to mechanical loading in vitro.</p><p><b>METHODS</b>MLO-Y4 osteocyte-like cells were exposed to fluid flow-induced shear stress(12dyn/cm(2))for 0, 1, 2, 4, 6, 12, and 24 hours. Osteocyte exposed to shear stress at different time points were used in co-culture system for 9 days, and then the cells were stained with tartrate-resistant acid phosphatase on the 9(th) day and the amount of positively stained osteoclasts were counted and compared. The expressions of osteoprotegerin (OPG) and receptor activator of nuclear factor-kappa (RANKL) were detected by semi-quantitative reverse transcription polymerase chain reaction (semi-quantitative RT-PCR).</p><p><b>RESULTS</b>Compared with bone cells without stimulation with fluid flow-induced shear stress, the amount of osteocytes significantly decreased at all time points after the application of fluid flow-induced shear stress (all P<0.05). The OPG expression at mRNA levels was significantly up-regulated in the first 12 hours (P<0.001), the RANKL mRNA expression was significantly down-regulated in the first 4 hours (P<0.05), and the RANKL/OPG ratio significantly decreased within 12 hours (P<0.01). However, all these indicators showed no significant difference at 24 hours when compared with the pre-stimulation level.</p><p><b>CONCLUSION</b>Osteocytes may act as mechanosensors that are able to inhibit bone resorption after mechanical loading; however, such effect shows certain adaptation ability to shear stress as time goes.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoclastes
/
Ostéocytes
/
Contrainte mécanique
/
Différenciation cellulaire
/
Cellules cultivées
/
Techniques de coculture
/
Biologie cellulaire
/
Ligand de RANK
/
Ostéoprotégérine
/
Métabolisme
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2012
Type:
Article
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