Inhibition of binding peptides on replication of duck hepatitis B virus / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 116-120, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-353235
ABSTRACT
<p><b>OBJECTIVE</b>To study the inhibitory effect of binding peptides on duck hepatitis B virus (DHBV) replication in duck hepatocytes.</p><p><b>METHODS</b>Specific binding peptides to duck hepatitis B virus polymerase (DHBVP) were screened by phage display technology (PDT), then were sequenced and synthesized. Binding peptides were added into primary culture of duck hepatocytes infected with DHBV in vitro. DHBV-DNA in the cytoplasm, cell nucleus and medium supernatant was assayed over time.</p><p><b>RESULTS</b>Seven binding peptides were obtained after 3-round screening by PDT. Duck primary hepatocytes infected by DHBV were treated with above obtained binding peptides. The DHBV-DNA levels in medium supernatant and cytoplasm of duck hepatocytes treated with synthesized peptides (the 3rd and the 6th peptide) were significantly lower than those of control cells (P<0.05).</p><p><b>CONCLUSION</b>Specific binding peptides to DHBVP could inhibit the replication of DHBV.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Virologie
/
Réplication virale
/
Virus de l'hépatite B du canard
/
Cellules cultivées
/
RNA-directed DNA polymerase
/
Infections à Hepadnaviridae
/
Acides nucléiques peptidiques
/
Hépatocytes
/
Canards
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2005
Type:
Article
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