Inhibition of HBV core antigen gene expression in human embryonic kidney cell line AD293 by plasmid-based RNAi / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To inhibit HBV core antigen gene expression with plasmid-based RNAi.</p><p><b>METHODS</b>The shRNA expression vector targeting HBV core antigen gene was designed and constructed. Human embryonic kidney cell line AD293 was co-transfected with HBcAg-EGFP fusion protein expression vector and shRNA expression vector transiently, and the cells without shRNA-transfection and with non-specific shRNA transfection were used as controls. Inhibitory effect of RNAi was detected by fluorescence-activated cell sorting (FACS) and real-time fluorescence quantificational RT-PCR.</p><p><b>RESULTS</b>HBV core antigen gene expression in AD293 was inhibited by shRNA, with the maximal inhibition rate of 76 % measured by FACS and of 63.1 % by real-time PCR.</p><p><b>CONCLUSION</b>Effective inhibition of HBV core antigen gene expression by plasmid-based RNAi provides an alternative for anti-HBV study in vitro, which has potential clinical application.</p>