Induction of HSF1 expression and sporadic colorectal cancer / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To explore the activation of the signal transduction pathways related with the carcinogenesis of sporadic colon cancers.</p><p><b>METHODS</b>A gene microarray monitoring activation of 8 signal transduction pathways (PathwayFinder GEArray) was used to screen the differentially expressed genes between colorectal cancer and normal colon tissue. The differentially expressed genes were further analyzed by RT-PCR, using RNA extracted from cancer tissue and matched normal colon mucosa of 35 patients with colorectal cancer.</p><p><b>RESULTS</b>The expression of hsf1, hsf27 and inos was increased in colon cancer compared with normal colon mucosa using PathwayFinder GEArray. The RT-PCR results showed that the expression of hsf1 was detected in 86% of patients(30/35)and the expression of inos detected in 63% patients(22/35).</p><p><b>CONCLUSION</b>Hsf1 induces heat shock stress signaling pathway, which might play a role in the carcinogenesis of sporadic colorectal cancer.</p>