Effect of astragalus polysaccharide on the function and maturation of plasmacytoid dendritic cells from chronic myelogenous leukemia before and after treatment / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 740-743, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-353557
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of astragalus polysaccharide (APS) on the function and maturation of chronic myelogenous leukemia (CML) peripheral blood mononuclear cells (PBMC)-derived plasmacytoid dendritic cells (pDCs).</p><p><b>METHODS</b>CML-derived pDCs were sorted by flow cytometry, and then incubated with APS (at 0, 50, 100 and 200 mg/L). After 24 hours, the concentrations of IFN-α, IL-6, TNF-α were detected with ELISA. Five days later, the cultured cells were collected and analyzed for immotype, morphology and ultramicrostructure.</p><p><b>RESULTS</b>The level of IFN-α, IL-6, TNF-α was significantly higher in samples from CML remission group than that in untreated pDCs, and newly diagnosed pDC (P < 0.05) or untreated group. APS could promote more pDCs differentiating to dendritic cells (DCs) in CML remission group than in untreated-pDCs in a dose-dependant manner (P < 0.05).</p><p><b>CONCLUSION</b>APS can enhance the immune function of pDCs, promote differentiation and maturation of pDCs from CML patients.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Polyosides
/
Cellules dendritiques
/
Agranulocytes
/
Leucémie myéloïde chronique BCR-ABL positive
/
Cellules cultivées
/
Interféron alpha
/
Allergie et immunologie
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2010
Type:
Article
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