Study of genetic susceptibility in 198 children with asthma / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 811-814, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-353859
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the frequency distribution of single nucleotide polymorphisms (SNPs) of four asthma-related gene loci (ACE I/D; ADRB2 Arg16Gly; TNF-α G-308A; MS4A2 Glu237Gly) in 198 asthmatic children, and to investigate its association with genetic susceptibility to childhood asthma and some clinical phenotypes of asthma.</p><p><b>METHODS</b>Polymerase chain reaction product electrophoresis identification and real-time quantitative PCR detecting system were used to determine the frequency distributions of the SNPs of the four asthma-related gene loci in 198 asthmatic children and 110 healthy controls. The serum total IgE (TIgE) levels and blood eosinophil proportion (%EOS) of the asthmatic children were measured. Different genotypes at the four asthma-related gene loci were compared in terms of TIgE and %EOS.</p><p><b>RESULTS</b>The genotype DD of angiotensin-converting enzyme (ACE) had a significantly higher frequency in the asthmatic children than in the healthy controls (χ2= 30.667, P<0.01), and the frequency of D allele was also significantly higher in the asthmatic children than in the healthy controls (χ2=7.151, P<0.01). No correlation was found between the polymorphism of each gene locus and serum TIgE level and %EOS (P>0.05).</p><p><b>CONCLUSIONS</b>Genotype DD of ACE is related to genetic susceptibility to childhood asthma and may be the risk factor for childhood asthma.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Asthme
/
Facteur de nécrose tumorale alpha
/
Récepteurs aux IgE
/
Récepteurs bêta-2 adrénergiques
/
Peptidyl-Dipeptidase A
/
Prédisposition génétique à une maladie
/
Polymorphisme de nucléotide simple
/
Génétique
/
Génotype
Type d'étude:
Étude pronostique
/
Facteurs de risque
Limites du sujet:
Enfant d'âge préscolaire
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Contemporary Pediatrics
Année:
2012
Type:
Article
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