Cloning of adriamycin-resistant related (arr) gene in an adriamycin-resistant L1210 variant
Experimental & Molecular Medicine
;
: 145-149, 1998.
Article
Dans Anglais
| WPRIM
| ID: wpr-35391
ABSTRACT
A partial fragment of novel sequence (arr, adriamycin-resistant related) was previously identified using the differential display (DD)-PCR technique with adriamycin-resistant L1210 variant (L1210AdR), which shows a typical multidrug resistant (MDR) phenotypes. The present research shows the isolation of full length arr cDNA sequence. To clone the full length cDNA of arr gene, DD-PCR fragments were subjected to 5'- and 3'-Rapid Amplification of cDNA End (RACE) method. The cloned arr cDNA consisted of 770 bases and contained an open reading frame of 153 bases, encoding a protein of 51 amino acid with the molecular mass of 4 kDa by in vitro translation reactions. Northern blot analysis showed that a 770 bases transcript arr gene was overexpressed in adriamycin-resistant L1210 variant, but not in the parent suggesting that the arr gene may be involved in the adriamycin-resistant phenotypes.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Biosynthèse des protéines
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Glycoprotéines
/
Leucémie L1210
/
Doxorubicine
/
Régulation de l'expression des gènes tumoraux
/
Technique de Northern
/
Séquence d'acides aminés
/
Clonage moléculaire
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Experimental & Molecular Medicine
Année:
1998
Type:
Article
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