Enhancing the efficiency of Epstein-Barr viral serologic test in the diagnosis of nasopharyngeal carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 356-359, 2002.
Article
Dans Anglais
| WPRIM
| ID: wpr-354024
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the efficiency of concurrent application of VCA-IgA, EA-IgA and EA-IgG serological tests in diagnosing nasopharyngeal carcinoma (NPC).</p><p><b>METHODS</b>The sera of 266 untreated NPC patients and 347 healthy adults were collected. In addition to the conventional immunoenzymatic method of VCA-IgA test, enzyme-linked immunosorbent assay (ELISA) was adopted as an alternative to test the antibody level of EA-IgG and EA-IgA. A new statistical formula was used to evaluate the odds ratio of different combinations of these three tests.</p><p><b>RESULTS</b>The sensitivity and specificity of VCA-IgA, EA-IgG and EA-IgA concurrently were as high as 95.11% and 97.41%, respectively, which were higher than those of single test (90.60% and 94.52% for VCA-IgA, 93.98% and 93.66% for EA-IgG, 89.84% and 88.18% for EA-IgA). Furthermore, the odds ratio of 3-test positivity (1 912.5) was higher than those of 2-test positivity (27.903 2 for VCA-IgA and EA-IgG, 11.169 0 for EA-IgG and EA-IgA, 8.032 8 for VCA-IgA and EA-IgA), which were even higher than those of 1-test positivity (0.121 4 for VCA-IgA, 0.170 5 for EA-IgG and 0.048 8 for EA-IgA).</p><p><b>CONCLUSION</b>ELISA is more accurate in reflecting the antibody level of EA-IgG and EA-IgA than the conventional immunoenzymatic method. The concurrent application of VCA-IgA, EA-IgG and EA-IgA test can markedly improve the sensitivity, specificity and odds ratio as well, thus resulting in enhancing the efficiency of diagnosing nasopharyngeal carcinoma serologically.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virologie
/
Sang
/
Immunoglobuline A
/
Tests sérologiques
/
Tumeurs du rhinopharynx
/
Sensibilité et spécificité
/
Herpèsvirus humain de type 4
/
Infections à virus Epstein-Barr
/
Protéines de capside
/
Diagnostic
Type d'étude:
Essai clinique contrôlé
/
Etude diagnostique
Limites du sujet:
Adulte
/
Humains
langue:
Anglais
Texte intégral:
Chinese Journal of Oncology
Année:
2002
Type:
Article
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