Radiation-inducible promoters-mediated cdglytk gene in the treatment of buccal carcinoma in golden hamster / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 549-552, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-354318
ABSTRACT
<p><b>OBJECTIVE</b>To observe the therapeutic effect of CDglyTK gene mediated by radiation-inducible promoters in the treatment of buccal carcinoma in Golden Hamster.</p><p><b>METHODS</b>Animal models of buccal carcinoma in golden hamster were established by painting 0.5% dimethyl-benzanthracene. The plasmids pcDNA (+) 3.1/E-CDglyTK were transfected into tumors by lipofectamine. 24 h later, the tumors were exposed to 3 Gy irradiation. Animals were monitored at regular intervals for volume of tumors. CDglyTK mRNA was assayed by RT-PCR. Apoptosis and proliferating cell nuclear antigen were detected respectively by in situ end-labeling and immunohistochemical methods.</p><p><b>RESULTS</b>Compared with control groups, the tumor was suppressed obviously by CDglyTK gene therapy combined with 3 Gy induction radiation. The expression of CDglyTK gene could be detected by RT-PCR in the transfected tumor, and up-regulation of CDglyTK expression was found in tumor exposed to radiation (P < 0.05). There was significant difference in apoptosis index or proliferation index between tumor without irradiation and tumor with irradiation (P < 0.05).</p><p><b>CONCLUSIONS</b>The radiation-inducible promoter can be served as a molecular switch to regulate the expression of CDglyTK gene in buccal carcinoma in golden hamster, and low dose induction radiation can significantly improve the therapeutic effects.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Effets des rayonnements
/
Radiothérapie
/
Thérapeutique
/
Thymidine kinase
/
Tumeurs de la bouche
/
Imagerie diagnostique
/
Carcinome épidermoïde
/
Radiographie
/
Thérapie génétique
/
Joue
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Stomatology
Année:
2006
Type:
Article
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