Characteristics of boningmycin induced cellular senescence of human tumor cells / 药学学报
Acta Pharmaceutica Sinica
; (12): 589-594, 2010.
Article
Dans Zh
| WPRIM
| ID: wpr-354586
Responsable en Bibliothèque :
WPRO
ABSTRACT
Cellular senescence is one of the important steps against tumor. This study was to observe the characteristics of boningmycin induced senescence of human tumor cells. MIT method and clone formation assay were used to detect the growth-inhibitory effect. Cellular senescence was detected with senescence-associated beta-galactosidase staining. Cell cycle distribution and accumulation of intracellular reactive oxygen species (ROS) were analyzed with flow cytometry. Protein expression was detected by Western blotting. The results showed that the growth-inhibitory effect of boningmycin was obviously stronger on human oral epithelial carcinoma KB cells than that on non-small cell lung cancer A549 cells. Comparison to the similar action of doxorubicin, that boningmycin induced the features of cellular senescence in both cell lines, its due to the arrest at G2/M phase and an increase of ROS level. The molecular senescence marker P21 increased significantly after boningmycin treatment at a dosage of 0.1 micromol x L(-1), whereas a higher concentration of it induced apoptosis. The results indicated that cellular senescence induced by boningmycin was one of its mechanisms in tumor suppression.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Pharmacologie
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Bléomycine
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Cellules KB
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Doxorubicine
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Cycle cellulaire
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Protéine p53 suppresseur de tumeur
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Vieillissement de la cellule
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Poly(ADP-ribose) polymerases
/
Espèces réactives de l'oxygène
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2010
Type:
Article