Proteomic analysis of mitochondrial proteins in hydroxycamptothecin-treated SMMC-7721 cells / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 572-576, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-354703
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differentially expressed mitochondrial proteins in hydroxycamptothecin (HCPT)-treated SMMC-7721 cells by comparative proteomic analysis.</p><p><b>METHODS</b>Apoptosis of SMMC-7721 cells were induced by using HCPT and their mitochondria were isolated with a mitochondria isolation kit for cultured cells. Three different solubility protein fractions were extracted with ReadyPrep Sequential Extraction Kit and were separated by two-dimensional gel electrophoresis (2-DE). PDQuest software was used to differentiate mitochondrial proteins between control cells and HCPT-treated cells. Matrix assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS) was used to identify some of the different proteins.</p><p><b>RESULTS</b>Highly purified mitochondria and high resolution 2-DE patterns of the proteins were obtained. Forty-four mitochondrial protein spots from the HCPT-treated cells showed different expressions compared to those of the control cells. Twenty of the different protein spots were analyzed by MALDI-TOF-MS.</p><p><b>CONCLUSION</b>Differently expressed mitochondrial proteins in HCPT-treated cells and control cells were obtained in this study. This will be of help to understand the mechanism by which HCPT induces cell apoptosis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Camptothécine
/
Protéines mitochondriales
/
Protéomique
/
Lignée cellulaire tumorale
/
Potentiels de membrane
/
Métabolisme
/
Antinéoplasiques d'origine végétale
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Hepatology
Année:
2007
Type:
Article
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