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Analysis on variation of hemagglutinin genes of sixteen H9N2 subtype avian influenza viruses isolated in Shandong area / 病毒学报
Chinese Journal of Virology ; (6): 272-277, 2012.
Article Dans Chinois | WPRIM | ID: wpr-354736
ABSTRACT
In order to explore the genetic mutations of the H9N2 subtype avian influenza viruses isolated in Shandong, sixteen avian influenza virus subtype H9N2 were isolated from different areas of Shandong Province. The complete HA fragments of the viruses were amplified by RT-PCR and the sequences were analyzed on homology and heredity evolution after the cloning and sequencing of the products. The results showed that the amino acid motif of cleavage sites for all the sixteen virus in the HA gene were RSSR decrease GLF, which was consistent with the characterization of the LPAIV. Seven to nine potential glycosylation sites were found during the analysis and the receptor binding sites were relatively conservative except the 198 site. The Leucine(L) at the amino acid position 234 in the HA genes of all isolates indicated the potential of binding with SAalpha,2-6 receptor of mammals. Homology analysis showed that the homology of HA nucleotide and amino acid sequences was 96.3%-99.9% and 97.1%-99.6% for different strains. They belonged to a branch of the A/Duck/Hong Kong/Y280/97 in the phylogenetic tree.
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phylogenèse / Variation génétique / Virologie / Oiseaux / Données de séquences moléculaires / Chimie / Chine / Séquence d'acides aminés / Classification / Similitude de séquences d'acides aminés Limites du sujet: Animaux Pays comme sujet: Asie langue: Chinois Texte intégral: Chinese Journal of Virology Année: 2012 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Phylogenèse / Variation génétique / Virologie / Oiseaux / Données de séquences moléculaires / Chimie / Chine / Séquence d'acides aminés / Classification / Similitude de séquences d'acides aminés Limites du sujet: Animaux Pays comme sujet: Asie langue: Chinois Texte intégral: Chinese Journal of Virology Année: 2012 Type: Article