Correlation between TCID50 and p27 antigen of avian leukosis virus subgroup J / 病毒学报
Chinese Journal of Virology
; (6): 521-525, 2011.
Article
de Zh
| WPRIM
| ID: wpr-354796
Bibliothèque responsable:
WPRO
ABSTRACT
To study the correlation between 50% tissue-culture infective dose (TCID50) value and p27 antigen S/P value of Avian leukosis virus subgroup J and discuss their significance, chicken embryo fibroblast (CEF) cells were inoculated with Avian leukosis virus subgroup J strain NX0101 and samples were tested continuously for ten days after changing maintenance media. The correlation between TCID50 and p27 antigen S/P value of ten days were then analysized. Simultaneously, DF-1 cells were inoculated with NX0101 and passaged to 20 generations. Samples taken from 1st generation, 5th generation, 10th generation, 15th generation and 20th generation were tested for the TCID50 titer and the p27 antigen S/P value separately. A significant Pearson correlation was found between them in CEF cells (r = 0.85277; P < 0.0001) and in DF-1 cells (r = 0.93000; P = 0.0220). This study provided an important parameter for predicting TCID50 by detecting the p27 antigen S/P value.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Virologie
/
Virulence
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Leucose aviaire
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Virus de la leucose aviaire
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Antigène nucléaire de prolifération cellulaire
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Charge virale
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Allergie et immunologie
/
Fibroblastes
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Virology
Année:
2011
Type:
Article