Detection of IgH-MMSET fusion gene in multiple myeloma patients and its significance / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 515-518, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-354832
ABSTRACT
<p><b>OBJECTIVE</b>To detect the IgH-MMSET fusion gene resulted from t (4;14) translocation in multiple myeloma and illuminate its significance.</p><p><b>METHODS</b>IgH-MMSET fusion gene was detected in bone marrow specimens of 25 multiple myeloma (MM) patients and MM cell line NCI-H929 using reverse-transcription PCR (RT-PCR) assay followed by nested PCR to increase the sensitivity. The purified PCR products were cloned into pGEM-T vector and then sequenced using M13 forward primers. The fragment sequences were compared with that in GenBank to find matched sequences.</p><p><b>RESULTS</b>Only a 438 base pair long fragment was obtained after RT-PCR assay and was confirmed by sequencing to be a fusion gene product of IgH gene and MMSET gene in MM cell line NCI-H929. The breakpoints were located within the C micro region of IgH gene on chromosome 14 and intron 3 of MMSET gene on chromosome 4. IgH-MMSET hybrid transcripts were detected in 3 of 25 MM patients through nested PCR assay. The amplified fragments of the 3 patients were 237 base pairs (bp), 239 bp and 239 bp in length, respectively. The breakpoints on chromosome 4 were identical to that of NCI-H929 cell.</p><p><b>CONCLUSIONS</b>The formation of IgH-MMSET fusion gene is resulted from t (4;14) translocation in MM. The incidence rate is 12.0%. The presence of IgH-MMSET fusion gene may predict poor prognosis.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Translocation génétique
/
Chromosomes humains de la paire 4
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Chromosomes humains de la paire 14
/
Protein-tyrosine kinases
/
Données de séquences moléculaires
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Séquence nucléotidique
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Protéines de fusion oncogènes
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Récepteur facteur croissance fibroblaste
/
RT-PCR
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Récepteur de type 3 des facteurs de croissance fibroblastique
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Adulte
/
Adulte très âgé
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2003
Type:
Article
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