Neuronal differentiation of PC12 cells induced by human periodontal ligament fibroblast-conditioned medium / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To examine whether human periodontal ligament fibroblasts (hPDLF) express neural growth factor (NGF).</p><p><b>METHODS</b>PC12 cells were cultured in hPDLF-conditioned medium to evaluate the secretion of NGF by hPDLF.</p><p><b>RESULTS</b>hPDLF-conditioned medium resulted in neuronal differentiation and neurite outgrowth from PC12 cells. Only a small percentage of the cells grown in anti-NGF antiserum and DMEM expressed neuritis.</p><p><b>CONCLUSION</b>hPDLF are capable of synthesizing NGF and secreting it into the medium as the signaling molecules for innervation during the healing of the periodontal tissue.</p>