Activity of the TNF-related apoptosis-inducing ligand gene expressed from the hTERT promoter on colon cancer cell line HT-29 / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 45-49, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-355147
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the expression and activity of TNF-related apoptosis-inducing ligand (TRAIL) gene expressed from the hTERT promoter on colon cancer cell line HT-29.</p><p><b>METHODS</b>GFP/TRAIL gene expressed from the hTERT promoter was transfected into HT-29 with adenoviral vectors system, expression and apoptosis inducing ability of GFP/TRAIL protein were determined with fluorescence-activated cell sorting (FACS) method.</p><p><b>RESULTS</b>The expression of GFP gene was 31.4 % and 67.0 % with either hTERT promoter or CMV promoter in DLD1 cells; GFP/TRAIL gene was able to inhibit cell growth (74.2%) and induce apoptosis (25.8%) of HT-29 cells. There was significant difference between Ad/hTERT-gTRAIL and the other two control groups (PBS and Ad/CMV-GFP, P<0.05).</p><p><b>CONCLUSION</b>The GFP/TRAIL gene with hTERT promoter transfected by adenoviral vector was successfully expressed in HT-29 cell, which can both inhibit cell growth and induce apoptosis of colon cancer cell line HT-29.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Physiologie
/
Cellules cancéreuses en culture
/
Adenoviridae
/
Régions promotrices (génétique)
/
Apoptose
/
Tumeurs du côlon
/
Telomerase
/
Cellules HT29
/
Protéines à fluorescence verte
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2006
Type:
Article
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