Alterations of myocardial ultrastructure and gene expression of calcium handling proteins in diabetic rat heart / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 454-458, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-355185
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the ultrastructure of myocardium and gene expression of calcium handling proteins in diabetic rat heart.</p><p><b>METHODS</b>Diabetes was induced in male Sprague-Dawley rats by a single injection of alloxanm (40 mg/kg ) and the rats in control group were injected with normal saline. At the end of 2, 4, 6 weeks after the induction of diabetes, the animals were sacrificed. The expression of calcium handling proteins was detected by reverse transcription-polymerase chain reaction (RT-PCR) and actin mRNA was used as internal standard. Heart tissue at the apex was obtained for light and electron microscope study.</p><p><b>RESULTS</b>At the end of 4 and 6 weeks, cardiosomatic ratio of diabetic rats was higher than that of control. Electron microscopy revealed a spectrum of subcellular remodeling in myocardium which was characterized by damaged myofibrils and mitochondria, dilated and swollen sarcoplasmic reticulum. Expression of phospholamban mRNAs was significantly increased, but 1,4,5-trisphosphate inositol receptor type 2, ryanodine receptor type 2 mRNAs were significantly decreased compared with those in the age-matched control rats. In contrast, the expression of sarco/endoplasmic reticulum Ca(2+)-ATPase mRNAs was not affected.</p><p><b>CONCLUSION</b>In diabetic rat heart, gene expression of calcium handling proteins was characterized by up-regulation of phospholamban and down-regulation of sarcoplasmic reticulum calcium release channel while electron microscopic analysis of myocardium revealed a spectrum of subcellular remodeling.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Réticulum sarcoplasmique
/
Protéines de liaison au calcium
/
ARN messager
/
Canaux calciques
/
Répartition aléatoire
/
Calcium
/
Rat Sprague-Dawley
/
Diabète expérimental
/
Réticulum endoplasmique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2005
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS