Identification of peptides binding to Pisum sativum agglutinin from a phage-displayed random peptide library / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 412-416, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-355193
ABSTRACT
<p><b>OBJECTIVE</b>To obtain peptides binding specifically to Pisum sativum agglutinin (PSA) from a phage-displayed random peptide library.</p><p><b>METHODS</b>(1) A phage-displayed random hexapeptide library was screened with PSA as target. (2) Dot blot was used to analyze the influence of the alpha-Met-D-mannoside on binding between PSA and phage-displayed peptides. (3) Three peptides (RMWSF, RYDYSY, LRLRQL) were selectively synthesized, and different concentrations were used to inhibit PSA and ConA binding to the HRP.</p><p><b>RESULTS</b>The enrichment occurred obviously after three rounds of screening. The insert sequences of amino acids, displayed on 22 phage DNAs from the third round of screening, were divided into three groups. The binding of phage-displayed peptides to PSA was specific as shown by dot blot and could be inhibited by alpha-Met-D-mannoside. LRLRQL was not dissolved in water. ARMWSF and RYDYSY inhibited binding of PSA to HRP, but failed to inhibit binding ConA to HRP.</p><p><b>CONCLUSION</b>The binding site of peptides ARMWSF and RYDYSY is different to that of alpha-Met-D-mannoside.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Peptides
/
Liaison aux protéines
/
Sites de fixation
/
Protéines recombinantes
/
Banque de peptides
/
Lectines végétales
/
Métabolisme
Type d'étude:
Essai clinique contrôlé
/
Etude diagnostique
langue:
Chinois
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2005
Type:
Article
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