Construction a lentiviral vector for RNA interference of glutathione peroxidase 2 gene and its effect on HepG2 cell apoptosis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 832-837, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-355274
ABSTRACT
<p><b>OBJECTIVE</b>To construct a RNA interference lentiviral vector for human glutathione peroxidase 2 (GPX2) gene and observe the effect of GPX2 knockdown on cell apoptosis.</p><p><b>METHODS</b>The sequence of the small interfering RNA (siRNA) for GPX2 interference was inserted into the pSicoR vector. HepG2 cells were infected by the packaged si-GPX2 lentivirus and the expression of GPX2 in the infected cells was detected by both RT-PCR and Western blotting. Changes of cell apoptosis following the infection were analyzed by flow cytometry.</p><p><b>RESULTS</b>The lentiviral particles pSicoR-GPX2 were successfully packaged. The expression of GPX2 in the infected cells was obviously down-regulated at both RNA and protein levels. GPX2 knockdown caused increased apoptosis rate, increased Bax expression and lowered Bcl-2 expression in HepG2 cells.</p><p><b>CONCLUSION</b>We have successfully constructed the lentiviral vector for RNA interference of human GPX2 gene.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Régulation négative
/
Apoptose
/
Lentivirus
/
Petit ARN interférent
/
Interférence par ARN
/
Cellules HepG2
/
Vecteurs génétiques
/
Génétique
/
Glutathione peroxidase
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2015
Type:
Article
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