Expression and functional assessment of solute carrier 26A transporter family in HEK-293 cells / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To express solute carrier 26A proteins in HEK-293 cells and explore their functions.</p><p><b>METHODS</b>SLC26A-eGFP plasmids were transiently transfected into HEK-293 cells, and the nonlinear capacitance of the cells expressing SLC26A proteins was measured by whole-cell patch recording.</p><p><b>RESULTS</b>All the SLC26A transporters were expressed on the membrane of HEK-293 cells. Each member of the SLC26A transporter family showed robust nonlinear capacitance, which represented their binding capability with anions.</p><p><b>CONCLUSION</b>The SLC26A transporters expressed on HEK cells show similar functions as expected in tissue environment. The plasmids we constructed facilitate structural and functional study of SLC26A transporters.</p>