Study on expression of cell surface L-selectin and soluble L-selectin in patients with acute leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 251-255, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-355671
ABSTRACT
To study the relationship between the level of the soluble L-selectin (sL-selectin) in plasma and surface L-selectin expression on leukemic cells and episode and state of illness in acute leukemia patients, the plasma level of sL-selectin was measured by a sandwich enzyme-linked immunosorbent assay, and the expressions of surface L-selectin and its gene (lyam-1) were detected by immunohistochemistry and RT-PCR. The results showed that the levels of sL-selectin were significantly higher in untreated and therapy-resistant acute leukemia patients, and expression of L-selectin mRNA and cell surface L-selectinin in untreated and NR patients were significantly lower than that in CR patients and control group (P < 0.05). The plasma levels of sL-selectin were significantly increased in patients with splenomegaly and hepatomegaly (extramedullary infiltration). The levels of sL-selectin were related to the clinical course of the acute leukemia patients. A significant correalation existed between expressions of L-selectin mRNA and surface L-selectin in acute leukemia patients (gamma = 0.782, P < 0.05). It is concluded that expression of L-selectin gene was down-regulated in level of mRNA and protein in acute leukemia patients and both changes were highly correlated. Monitoring of the plasma level of sL-selectin is possibly useful for early diagnosis of relapse and extramedullary infiltration in acute leukemia.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Solubilité
/
Sang
/
ARN messager
/
Cellules de la moelle osseuse
/
Agranulocytes
/
Leucémie aigüe myéloïde
/
Régulation de l'expression des gènes tumoraux
/
Sélectine L
/
RT-PCR
Type d'étude:
Étude de dépistage
Limites du sujet:
Adolescent
/
Adulte
/
Adulte très âgé
/
Enfant
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2003
Type:
Article
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