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Cultivation of human mesenchymal stem cells on macroporous CultiSpher G microcarriers / 中国实验血液学杂志
Article de Zh | WPRIM | ID: wpr-355725
Bibliothèque responsable: WPRO
ABSTRACT
Adult human bone marrow-derived mesenchymal stem cells (MSCs) were cultured on microcarriers in spinner flasks and were compared with those in conventional culture in 12-well plates. For the production of adherently growing MSCs, macroporous CultiSpher G gelatin microcarriers were used in concentration of 1 g/L. The cells were seeded in a density of 5 x 10(4) cells/ml in both spinner culture and conventional stationary culture. The result showed that after 7 days of cultivation a maximum viable cell concentration of 5.15 x 10(5) cells/ml was obtained in spinner culture. Whereas the cell density increased to a maximum of 1.675 x 10(5) cells/ml on day 5 in conventional stationary culture. Lactate was produced up to 12.06 mmol/L in spinner culture and up to 13.10 mmol/L in stationary culture, and glucose was consumed up to 7.38 mmol/L and 5.37 mmol/L respectively. The average lactate yield on glucose consumption in spinner culture was only 1.63, lower than that in stationary culture 2.44. This indicated that the energy metabolism in spinner culture was significantly more efficient than that in conventional culture. After spinner culture for 12 days, the MSCs maintain the characteristics of stem cells. It is concluded that the microcarrier culture system is a suitable way to expand the seeding cells for tissue engineering.
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Cellules souches / Facteurs temps / Cellules de la moelle osseuse / Microscopie électronique / Microscopie électronique à balayage / Antigènes HLA-DR / Antigènes CD / Numération cellulaire / Division cellulaire / Survie cellulaire Limites du sujet: Adult / Humans langue: Zh Texte intégral: Journal of Experimental Hematology Année: 2003 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Cellules souches / Facteurs temps / Cellules de la moelle osseuse / Microscopie électronique / Microscopie électronique à balayage / Antigènes HLA-DR / Antigènes CD / Numération cellulaire / Division cellulaire / Survie cellulaire Limites du sujet: Adult / Humans langue: Zh Texte intégral: Journal of Experimental Hematology Année: 2003 Type: Article