Simultaneous determination of 6 active components in Chrysanthemum morifolium by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1474-1477, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-356094
ABSTRACT
<p><b>OBJECTIVE</b>To develop a HPLC method quantitative method for simultaneous determination of chlorogenic acid, 1, 5-dicaffeoylquinic acid, isochlorogenic acid A, isochlorogenic acid C, luteolin-7-O-beta-D-glucoside and apigenin-7-O-beta-D-glucoside in Chrysanthemum morifolium Ramat.</p><p><b>METHOD</b>A Phenomenex Gemini-NX C18 column (4.6 mm x 250 mm, 5 microm) was used with CH3 OH and 0.4% H3PO4 as mobile phases. The flow rate was 1 mL x min(-1), the column temperature was 25 degrees C, and the detection wavelength was set at 350 nm.</p><p><b>RESULT</b>The 6 active components were in baseline separation. The linearity of this method was good (r > or = 0.999 7), and the average recoveries were 100.6% - 102.4%, RSD < 3%. Except isochlorogenic acid A, the contents of the determined components in the steam-blanched flower heads were significantly higher than those non blanched. The contents of chlorogenic acid and isochlorogenic acid A in the steam-blanched semiopened flower heads were higher than fully opened ones by 53% and 41%, respectively.</p><p><b>CONCLUSION</b>The method is sensitive, accurate, reliable and repeatable, which can be used for quality evaluation of Chrysanthemum.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Contrôle de qualité
/
Extraits de plantes
/
Chimie
/
Acide chlorogénique
/
Reproductibilité des résultats
/
Chromatographie en phase liquide à haute performance
/
Sensibilité et spécificité
/
Cinnamates
/
Chrysanthemum
/
Fleurs
Type d'étude:
Etude diagnostique
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2011
Type:
Article
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