Effects of human HSF1 on transcriptional regulation of prohibitin promoter / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 349-352, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-356256
ABSTRACT
<p><b>AIM</b>To assay the transcriptional regulation effect of hHSF1 on prohibitin gene promoter.</p><p><b>METHODS</b>The total length of hHSF1 exon was amplified by PCR method and cloned into pcDNA3. 1(+) vector. pcDNA3. 1(+)-hHSF1 and pGI3 prohibitin were co-transfected into HEK293 cells. The luciferase activity was detected by Dual-Luciferase Reporter Assay System to evaluate the transcriptional regulation effect of hHSF1 on prohibitin gene promoter.</p><p><b>RESULTS</b>The pcDNA3.1(+)-hHSF1 vector was constructed successfully. The assay of luciferase activity showed that the transcription of pGL3-prohibitin was dramatically upregulated by hHSF1.</p><p><b>CONCLUSION</b>hHSF1 can transcriptionally regulate prohibitin expression.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Protéines de répression
/
Facteurs de transcription
/
Transcription génétique
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Transfection
/
Régulation de l'expression des gènes
/
Régions promotrices (génétique)
/
Protéines de liaison à l'ADN
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2009
Type:
Article
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