Changes of leptin levels in serum and myocardium after myocardial ischemia/reperfusion injury / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 221-223, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-356291
ABSTRACT
<p><b>AIM</b>To explore the effect of rat myocardial ischemia/reperfusion injury on leptin levels in serum and myocardium, and discuss the role of leptin in myocardial ischemia/reperfusion injury.</p><p><b>METHODS</b>A myocardial ischemia/reperfusion injury model of rats was established, serum lactate dehydrogenase (LDH) and leptin levels were detected, and histopathological changes and leptin expressions in myocardium were investigated by hematoxylin-eosin staining and immunohistochemistry, respectively.</p><p><b>RESULTS</b>Serum LDH of ischemia and reperfusion groups increased significantly (P < 0.05), suggesting the model was successfully established and a certain degree of local myocardial injury was induced. Serum leptin of ischemia group (6.34 +/- 2.49) ng/ml was significantly lower than control group (7.50 +/- 2.93 ng/ml, P <0.05). Leptin levels recovered gradually after reperfusion, reached (8.32 +/- 1.74)ng/ml at 2 h after reperfusion, which recovered to the level before injury (8.38 +/- 2.56) ng/ml, and showed a trend to increase as reperfusion time was elongated. Immunohistochemistry results showed that as compared with sham-operation group, myocardial leptin protein expressions of the other four groups were all significantly lower (P < 0.01), and decreased in order by 45 min ischemia/1 h reperfusion, 45 min ischemia/3 h reperfusion, 45 min ischemia and 45 min ischemia/2 h reperfusion.</p><p><b>CONCLUSION</b>Leptin level in the blood decreases significantly at the early 45 min after myocardial ischemia/reperfusion injury, and its expression in myocardium also decreases significantly. There may be a certain relationship between the pathological injury of myocardium and the changes of leptin.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Sang
/
Lésion de reperfusion myocardique
/
Répartition aléatoire
/
Rat Sprague-Dawley
/
Leptine
/
L-Lactate dehydrogenase
/
Métabolisme
/
Myocarde
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2009
Type:
Article
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