Rapid detection of novel avian influenza virus subtype H7N9 by multiplex real-time RT-PCR / 病毒学报
Chinese Journal of Virology
;
(6): 1-5, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-356647
ABSTRACT
In order to develop a rapid detection kit for novel avian influenza virus (AIV) subtype H7N9, two sets of specific primers and probes were designed based on the nucleotide sequences of hemagglutinin antigen (HA) and neuraminidase antigen (NA) of novel H7N9 virus (2013) available in GenBank to establish the method of TaqMan probe-based multiplex real-time RT-PCR for rapid detection of AIV subtype H7N9. The primer and probe of HA were for all H7 subtype AIVs, while the primer and probe of NA were only for novel N9 subtype AIVs. The results showed that this method had high sensitivity and specificity. This method was applicable to the testing of positive standard sample with a minimum concentration of 10 copies/microL; it not only distinguished H7 subtype from H1, H3, H5, H6, and H9 subtypes, but also distinguished novel N9 subtype from traditional N9 subtype. A total of 2700 samples from Zhuhai, China were tested by this method, and the results were as expected. For the advantages of sensitivity and specificity, the method holds promise for wide application.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Spécificité d'espèce
/
Facteurs temps
/
Virologie
/
Oiseaux
/
TAQ polymerase
/
Réaction de polymérisation en chaine en temps réel
/
Sous-type H7N9 du virus de la grippe A
/
Grippe chez les oiseaux
/
Génétique
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Virology
Année:
2014
Type:
Article
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