Genomic characteristics of coxsackievirus B1 MSH/KM9/2009 strain isolated in Yunnan, China / 病毒学报
Chinese Journal of Virology
; (6): 293-298, 2013.
Article
Dans Zh
| WPRIM
| ID: wpr-356689
Responsable en Bibliothèque :
WPRO
ABSTRACT
To characterize the complete genome sequence of coxsackievirus B1 (CVB1) MSH/KM9/2009 strain isolated from Yunnan, China,2009. Eight overlapping clones covering the whole viral genome (excluding the poly-A tail) were obtained by RT-PCR and sequenced, and their nucleotide and amino acid sequences were compared with other known CVB1 strains. The genome of the CVB1 MSH/KM9/2009 strain had 7384 nucleotides in length, and contained a 741nt non-translated region (NTR) at the 5' end and a 94nt NTR at the 3' end. The entire open reading frame contained 6 549 nt, encoding a 2 183-aa polyprotein. In the coding region, there was no nucleotide deletion or insertion, but some changes of amino acid were unique. The complete genome sequence alignments showed that the CVB1 isolate MSH/KM9/2009 strain shared the highest nucleotide (80.9%, 81.6%, 80.5% and 80%) and amino acid (95.6%, 95.8%, 96.2% and 95.6%) identities to the CVB1 M16560, pmMC, Tucson B1 and CVB1Nm strain, respectively. Phylogenetic tree analysis showed that the MSH/KM9/2009, CVB1 M16560, pmMC, Tucson B1 and CVB1Nm strain clustered into same group. The newly isolated CVB1 strain MSH/KM9/2009 from Yunnan Province belonged to genotype CVB1.
Texte intégral:
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Indice:
WPRIM
Sujet Principal:
Phylogenèse
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Protéines virales
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Virologie
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Séquence nucléotidique
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Chine
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Cadres ouverts de lecture
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Classification
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Génome viral
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Enterovirus
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Infections à virus coxsackie
Limites du sujet:
Child, preschool
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Female
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Humans
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Infant
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Male
Pays comme sujet:
Asia
langue:
Zh
Texte intégral:
Chinese Journal of Virology
Année:
2013
Type:
Article