Fingerprint analysis of composite Folii Isatidis injection by HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop the chromatographic fingerprints of composite Folii Isatidis injection by HPLC.</p><p><b>METHOD</b>The separation was performed on a Diamonsil C18 column with a mobile phase consisting of methanol-water-phosphoric acid as gradient eluent at the flow rate of 1.0 mL x min(-1). The UV detection was set at 254 nm.</p><p><b>RESULT</b>A standard HPLC fingerprint procedure was developed for composite Folii Isatidis injection, with 20 common peaks and a similarity threshold of 9.0 established.</p><p><b>CONCLUSION</b>This method was accurate, repeatable and useful for the quality control of composite Folii Isatidis injection.</p>