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Major constituent proteins in donkey hide and their interaction / 中国中药杂志
Article de Zh | WPRIM | ID: wpr-356749
Bibliothèque responsable: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the constituent proteins in donkey hide, the key ingredient for Ejiao, an important traditional Chinese medicine for the blood-related conditions, in hope to eventually decipher the biochemical mechanism behind Ejiao's prominent medicinal efficacy.</p><p><b>METHOD</b>Two methods were employed to extract proteins in donkey skin. One used TriPure isolation reagent to extract the total proteins in donkey skin. Another used 1% sodium dodecyl sulfate (SDS) to heat the sample at 100 degrees C overnight. And then sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and capillary HPLC were used to analyze the component of proteins.</p><p><b>RESULT</b>There are not only collagen alpha1 (I) and collagen alpha2 (I), but also serum albumin in donkey skin. The content is over 25% in total proteins with the method of TriPure isolation reagent. The content of donkey serum albumin is up to 20% with the method of 1% SDS heating. And two bands, molecular weight are nearly 200 kDa,were found on 7.5% SDS-PAGE. Extracted these proteins to analyze with capillary HPLC, they were found to be the complex products of collagen and serum albumin of donkey.</p><p><b>CONCLUSION</b>Donkey serum albumin is a main protein component in the hide, which is a clue to expose is the effect of Ejiao on blood.</p>
Sujet(s)
Texte intégral: 1 Indice: WPRIM Sujet Principal: Liaison aux protéines / Peau / Sérumalbumine / Chimie / Chromatographie en phase liquide à haute performance / Equidae / Collagène de type I / Collagène de type II / Interactions médicamenteuses / Électrophorèse sur gel de polyacrylamide Limites du sujet: Animals langue: Zh Texte intégral: China Journal of Chinese Materia Medica Année: 2006 Type: Article
Texte intégral: 1 Indice: WPRIM Sujet Principal: Liaison aux protéines / Peau / Sérumalbumine / Chimie / Chromatographie en phase liquide à haute performance / Equidae / Collagène de type I / Collagène de type II / Interactions médicamenteuses / Électrophorèse sur gel de polyacrylamide Limites du sujet: Animals langue: Zh Texte intégral: China Journal of Chinese Materia Medica Année: 2006 Type: Article