Effect of As₂O₃on Hedgehog Pathway in Chronic Myeloid Leukemia Cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 971-975, 2015.
Article
de Zh
| WPRIM
| ID: wpr-357236
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of As₂O₃on Hedgehog pathway in chronic myeloid leukemia (CML) cells.</p><p><b>METHODS</b>The apoptosis of K562 cells was detected by MTT method and flow cytometry; the expressions of PTCH and SMO protein and mRNA in Hedgehog pathway were determined by Western blot and real-time PCR, respectively. Retults: The As₂O₃ could induce the apoptosis of K562 cells with optimal concentration 2 µmol/L and optimal time 24 hours. The expressions of PTCH and SMO protein and mRNA in Hedgehog pathway of K562 cells treated with As₂O₃at optimal concentration and optimal time were down-regulated.</p><p><b>CONCLUSION</b>The As₂O₃can down-regulate the expression of PTCH and SMO in Hedgehog pathway.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Oxydes
/
Composés de l'arsenic
/
ARN messager
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Leucémie myéloïde chronique BCR-ABL positive
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Transduction du signal
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Régulation négative
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Apoptose
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Cellules K562
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Protéines Hedgehog
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Réaction de polymérisation en chaine en temps réel
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Journal of Experimental Hematology
Année:
2015
Type:
Article