Insulin/PI3K signalling pathway regulates the expression of survivin in liver cancer HepG2 cells / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 745-748, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-357348
ABSTRACT
<p><b>OBJECTIVE</b>To determine the expression level changes of survivin, a inhibitor of apoptosis protein, followed by activation of insulin receptors in human hepatocellular carcinoma HepG2 cell line, and to investigate the signalling pathway involved in the regulation.</p><p><b>METHODS</b>Human hepatocellular carcinoma HepG2 cells were treated with insulin alone or pre-treated with LY294002, a specific inhibitor of PI3K signalling pathway, to determine whether blocking PI3K signaling can attenuate the up-regulation of survivin expression. Real time RT-PCR and Western blot analysis were used to measure survivin mRNA and protein changes before and after treatment, respectively.</p><p><b>RESULTS</b>Without serum supplement, HepG2 cells expressed a small amount of survivin. Insulin induced survivin expression in a dose- and time-dependent fashion. Survivin expression was blocked if cells were pre-treated with LY294002 prior to insulin stimulation.</p><p><b>CONCLUSION</b>Insulin induces survivin expression via PI3K signalling pathway, suggesting that to interfere the key gene in this signalling pathway may block survivin expression, therefore, promoting apoptosis in hepatocellular carcinoma cells.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
ARN messager
/
Transduction du signal
/
Régulation de l'expression des gènes tumoraux
/
Régulation positive
/
Morpholines
/
4H-1-Benzopyran-4-ones
/
Apoptose
/
Phosphatidylinositol 3-kinases
/
Relation dose-effet des médicaments
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Oncology
Année:
2008
Type:
Article
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