Effect of microRNA143 expression on cell proliferation in colonic carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 498-501, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-357389
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of microRNA143 on cell proliferation and K-ras expression in colorectal carcinoma.</p><p><b>METHODS</b>Northern blot was used to examine the expression of miR-143 in colorectal carcinoma and adjacent normal tissues. A miR-143 expression vector was constructed and transfected into a human colon adenocarcinoma cell line SW480. Cell proliferation was evaluated by MTT assay. RT-PCR and Western blot were used to examine the expression of K-ras oncogene in transfected cells.</p><p><b>RESULTS</b>The level of mature miR-143 was lower in tumors compared with adjacent normal tissues in 81% of colorectal carcinoma specimens. In transfected cells, the increased accumulation of miR-143 inhibited the cell proliferation, and resulted in approximately 40.3% decrease of K-ras protein levels, but had no effect on level of K-ras mRNA.</p><p><b>CONCLUSION</b>The increased accumulation of miR-143 inhibits the proliferation of transfected cells, and results in down-regulation of K-ras protein in colorectal carcinoma.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Plasmides
/
ARN messager
/
Transfection
/
Adénocarcinome
/
Régulation négative
/
Gènes ras
/
Tumeurs du côlon
/
Protéines G ras
/
MicroARN
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Oncology
Année:
2008
Type:
Article
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