ITS sequence identification of Radix Bupleuri / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 732-734, 2005.
Article
de Zh
| WPRIM
| ID: wpr-358123
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To provide the basis of molecular authentication of Radix Bupleuri by the comparison of the internal transcribed spacer (ITS) sequences of five kinds of Radix Bupleuri in common use.</p><p><b>METHOD</b>Firstly, total DNA of five kinds of Radix Bupleuri was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and PCR product was directly sequenced after purification.</p><p><b>RESULT</b>The length of ITS1 and ITS2 sequence was 214-220 bp and 230-231 bp respectively. There were distinct variation sites between the ITS sequences of the five kinds of Radix Bupleuri.</p><p><b>CONCLUSION</b>ITS sequence may be the evidence for the molecular authentication of Radix Bupleuri.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Phylogenèse
/
Plantes médicinales
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Classification
/
Racines de plante
/
ADN des plantes
/
Espaceur de l'ADN ribosomique
/
Bupleurum
/
Cuminum
Type d'étude:
Diagnostic_studies
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2005
Type:
Article