Expression of IgVH and B7-1 in proteome of the human colorectal carcinoma cell lines / 中华肿瘤杂志
Zhonghua zhong liu za zhi
; (12): 648-652, 2005.
Article
de Zh
| WPRIM
| ID: wpr-358545
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To conduct a proteomic analysis of human colorectal carcinoma cell lines LS174T and SW480 by two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS).</p><p><b>METHODS</b>The total proteins of human colorectal carcinoma cell lines LS174T and SW480 were separated with 2-DE using immobilized pH gradient strips and analyzed by MALDI-TOF-MS to obtain peptide mass fingerprints (PMFs). Proteins were identified by using Mascot software to search protein databases.</p><p><b>RESULTS</b>Good resolution 2-DE maps were obtained. Some proteins including immunoglobulin heavy chain variable region (IgVH) and co-stimulatory molecule B7-1 were identified. IgVH and B7-1 were confirmed by electrospray ionization tandem spectrometry (ESI-MS/MS) and immunocytochemistry.</p><p><b>CONCLUSION</b>There are IgVH and B7-1 expressions in human colorectal carcinoma cell lines LS174T and SW480. Results obtained will help to elucidate the mechanisms of tumor immune escape.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Cartographie peptidique
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Région variable d'immunoglobuline
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Lignée de cellules transformées
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Tumeurs colorectales
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Chaines lourdes des immunoglobulines
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Antigène CD80
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Protéome
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Allergie et immunologie
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Génétique
/
Métabolisme
Type d'étude:
Prognostic_studies
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
Zhonghua zhong liu za zhi
Année:
2005
Type:
Article