Effects of TOLL-like receptor 4 on passively sensitized human airway smooth muscle cells proliferation and synthesis and secretion function of TGF-beta1 / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 20-24, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-358689
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the activation of Toll like receptor 4 (TLR4) on passively sensitized human airway smooth muscle cells (HASMCs) proliferation and the synthesis and secretion function.</p><p><b>METHODS</b>Through the cultivation of primary HASMCs, we studied TLR4 expression on cell surface, cell proliferation and transformation of parturient factor-beta1 (TGF-beta1) in asthma under the condition of synthesis and secretion level by passively sensitized HASMCs with asthma serum.</p><p><b>RESULTS</b>Compared with the control group, in passive sensitized group and TNF-alpha group TLR4 expression were significantly increased (P < 0.01), significantly enhanced proliferation (P < 0.01), total protein concentration, IgE secretion and TGF-beta1 were significantly higher (P < 0.01); and all the above parameters were increased more significantly in TNF group compared with those in the target effect of passively group; and those parameters were significantly reduced in anti-TLR4 antibody group compared with those in the target effect both of passively sensitized group and TNF-alpha group.</p><p><b>CONCLUSION</b>TLR4 on passively sensitized HASMCs activated can induce the excessive proliferation of HASMCs and a large number of synthesis and secretion of TGF-beta1, resulting in changing airway micro-environment, which involved in airway remodeling in asthma.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Asthme
/
Bronches
/
Cellules cultivées
/
Biologie cellulaire
/
Myocytes du muscle lisse
/
Prolifération cellulaire
/
Allergie et immunologie
/
Récepteur de type Toll-4
/
Facteur de croissance transformant bêta-1
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2013
Type:
Article
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