Effect of osteoprotegerin in combination with interleukin-6 on inhibition of osteoclast differentiation / 中华创伤杂志(英文版)
Chinese Journal of Traumatology
;
(6): 277-280, 2013.
Article
Dans Anglais
| WPRIM
| ID: wpr-358935
ABSTRACT
<p><b>OBJECTIVE</b>To observe the effect of recombinant interleukin-6 (IL-6) and osteoprotegerin (OPG) on inhibiting bone absorption induced by receptor activator for nuclear factor-kB ligand (RANKL) in murine osteoclast precursor cells (OCPs) model.</p><p><b>METHODS</b>RAW 264.7 cells were solely treated with 50 ng/ml RANKL for 1 day, and then they were divided into three groups RANKL (control group), RANKL+IL-6 (IL-6 group) and RANKL+IL-6+OPG (combination group). These cells were harvested and investigated by means of HE staining under light microscope after consecutive 9 days. Furthermore, staining tartrate-resistant acid phosphatase(TRAP)-positive multinucleated cells were detected by inverted phase contrast microscope. The absorption pits of bone slices were observed under scanning electron microscope.</p><p><b>RESULTS</b>The number of mature osteoclast cells in control group was more than that in IL-6 alone or IL-6 combined with OPG group (P less than 0.05). Interestingly, this experiment has also demonstrated that there was a large number of TRAP-positive multinucleated osteoclasts (more than 3 nuclei) and several bone absorption formation in the control group, whereas the outcome was completely different in both IL-6 group and IL-6+OPG group (P less than 0.05).</p><p><b>CONCLUSION</b>IL-6 can suppress the differentiation of mature osteoclasts as directly adding it into the RAW 264.7 cells induced by 50 ng/ml RANKL, and further the effect of osteolysis is remarkably reduced. When treatment with IL-6 combined with OPG, a more effective strategy for the treatment of osteoporosis is reached.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoclastes
/
Pharmacologie
/
Protéines recombinantes
/
Différenciation cellulaire
/
Cellules cultivées
/
Interleukine-6
/
Biologie cellulaire
/
Ligand de RANK
/
Ostéoprotégérine
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Chinese Journal of Traumatology
Année:
2013
Type:
Article
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