Protection against acute hypoxic/reoxygenation injury to kidney for rabbit with morphine hypoxic preconditioning by observing the expression of caspase-3 protein / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 531-533, 2011.
Article
de Zh
| WPRIM
| ID: wpr-359231
Bibliothèque responsable:
WPRO
ABSTRACT
The maintenance of the balance between oxygen supply and oxygen consumption is a key measure in preventing acute kidney hypoxic/reoxygenation injury. Morphine can inhibit metabolism and reduce the oxygen consumption. We tried to investigate the protective effects of morphine hypoxic preconditioning on acute kidney hypoxic/reoxygenation injury in rabbit and its influence on expression of caspase-3 protein. Kidney hypoxic and reoxygenation were induced by making the tested rabbits inhale 8% oxygen for three hours firstly, and then putting them in the air to breathe in normal oxygen for another three hours. Morphine hypoxic preconditioning was induced by administering morphine 3 mg/kg, and then hypoxic of 8% oxygen was induced. Caspase-3 protein expression in renal tissue was assessed by immunohistochemical method. In the present study, the expressions of caspase-3 protein were significantly higher in saline-control hypoxic group than in morphine hypoxic preconditioning group ((29.3+/-5.7)% vs. (12.16+1.23)%, P<0.05). These observations suggested that morphine hypoxic preconditioning can protect rabbit against acute kidney hypoxic/reoxygenation injury by decreasing expression of caspase-3 protein.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Pharmacologie
/
Superoxide dismutase
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Lésion d'ischémie-reperfusion
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Répartition aléatoire
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Préconditionnement ischémique
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Caspase-3
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Rein
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Métabolisme
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Méthodes
/
Morphine
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Journal of Biomedical Engineering
Année:
2011
Type:
Article