Albumin resuscitation protects against traumatic/hemorrhagic shock-induced lung apoptosis in rats / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
;
(12): 871-878, 2008.
Article
Dans Anglais
| WPRIM
| ID: wpr-359347
ABSTRACT
<p><b>OBJECTIVE</b>To determine the effects of albumin administration on lung injury and apoptosis in traumatic/hemorrhagic shock (T/HS) rats.</p><p><b>METHODS</b>Studies were performed on an in vivo model of spontaneously breathing rats with induced T/HS; the rats were subjected to femur fracture, ischemia for 30 min, and reperfusion for 20 min with Ringer's lactate solution (RS) or 5% (w/v) albumin (ALB), and the left lower lobes of the lungs were resected.</p><p><b>RESULTS</b>Albumin administered during reperfusion markedly attenuated injury of the lung and decreased the concentration of lactic acid and the number of in situ TdT-mediated dUTP nick-end labelling (TUNEL)-positive cells. Moreover, immunohistochemistry performed 24 h after reperfusion revealed increases in the level of nuclear factor kappaB (NF-kappaB), and phosphorylated p38 mitogen-activated protein kinase (MAPK) in the albumin-untreated group was down-regulated by albumin treatment when compared with the sham rats.</p><p><b>CONCLUSION</b>Resuscitation with albumin attenuates tissue injury and inhibits T/HS-induced apoptosis in the lung via the p38 MAPK signal transduction pathway that functions to stimulate the activation of NF-kappaB.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Oxygène
/
Anatomopathologie
/
Réanimation
/
Choc hémorragique
/
Sang
/
Immunohistochimie
/
Lésion d'ischémie-reperfusion
/
Répartition aléatoire
/
Rat Sprague-Dawley
/
Apoptose
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Journal of Zhejiang University. Science. B
Année:
2008
Type:
Article
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