Adriamycin induces H2AX phosphorylation in human spermatozoa / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
; (6): 749-757, 2008.
Article
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| WPRIM
| ID: wpr-359914
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ABSTRACT
<p><b>AIM</b>To investigate whether adriamycin induces DNA damage and the formation of gammaH2AX (the phosphorylated form of histone H2AX) foci in mature spermatozoa.</p><p><b>METHODS</b>Human spermatozoa were treated with adriamycin at different concentrations. gammaH2AX was analyzed by immunofluorescent staining and flow cytometry and double-strand breaks (DSB) were detected by the comet assay.</p><p><b>RESULTS</b>The neutral comet assay revealed that the treatment with adriamycin at 2 microg/mL for different times (0.5, 2, 8 and 24 h), or for 8 h at different concentrations (0.4, 2 and 10 microg/mL), induced significant DSB in spermatozoa. Immunofluorent staining and flow cytometry showed that the expression of gH2AX was increased in a dose-dependent and time-dependant manner after the treatment of adriamycin. Adriamycin also induced the concurrent appearance of DNA maintenance/repair proteins RAD50 and 53BP1 with gammaH2AX in spermatozoa. Wortmannin, an inhibitor of the phosphatidylinositol 3-kinase (PI3K) family, abolished the co-appearance of these two proteins with gammaH2AX.</p><p><b>CONCLUSION</b>Human mature spermatozoa have the same response to DSB-induced H2AX phosphorylation and subsequent recruitment of DNA maintenance/repair proteins as somatic cells.</p>
Texte intégral:
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Indice:
WPRIM
Sujet Principal:
Pharmacologie
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Phosphorylation
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Spermatozoïdes
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Altération de l'ADN
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Histone
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Doxorubicine
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Cellules cultivées
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Test des comètes
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Biologie cellulaire
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Enzymes de réparation de l'ADN
Limites du sujet:
Humans
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Male
langue:
En
Texte intégral:
Asian Journal of Andrology
Année:
2008
Type:
Article