Effect of glycyrrhetinic acid on invasive capacity of leukemic cells and activity of gelatinase / 中国中西医结合杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of glycyrrhetinic acid (GA) on the invasive capacity of leukemic cells and the activity of intracellular gelatinase.</p><p><b>METHODS</b>The effect of GA, in different concentrations, on the proliferation of cultured K562 and HL-60 leukemic cells in vitro was determined by MTT assay; that on cell invasive capacity was tested by Transwell cubicle matrigel invasion assay; and that on the activity of gelatinase in cells was detected by gelatin zymography.</p><p><b>RESULTS</b>GA showed significant inhibitory effect on the proliferation of K562 and HL-60 leukemic cells; it inhibited the invasive capacity of cells in concentration-dependent manner; and significantly down-regulated the activity of gelatinase A and B in cells.</p><p><b>CONCLUSIONS</b>GA can inhibit invasive capacity of K562 and HL-60 leukemia cells by way of suppressing the activity of gelatinase A and B. This study provides an experimental evidence for preventing extra-medullary infiltration of leukemic cells.</p>