Alternative expression and sequence of human elongation factor-1 delta during malignant transformation of human bronchial epithelial cells induced by cadmium chloride / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 151-157, 2010.
Article
Dans Anglais
| WPRIM
| ID: wpr-360610
ABSTRACT
<p><b>OBJECTIVE</b>To study the alternative expression and sequence of human elongation factor-1 delta (human EF-1 delta p31) during malignant transformation of human bronchial epithelial cells induced by cadmium chloride (CdC12) and its possible mechanism.</p><p><b>METHODS</b>Total RNA was isolated at different stages of transformed human bronchial epithelial cells (16HBE) induced by CdCl2 at a concentration of 5.0 microM. Special primers and probe for human EF-1 delta p31 were designed and expression of human EF-1 delta mRNA from different cell lines was detected with fluorescent quantitative PCR technique. EF-18 cDNA from different cell lines was purified and cloned into pMD 18-T vector followed by confirming and sequencing analysis.</p><p><b>RESULTS</b>The expressions of human EF-1 beta p31 at different stages of 16HBE cells transformed by CdCl2 was elevated (P < 0.01 or P < 0.05). Compared with their corresponding non-transformed cells, the overexpression level of EF-1 delta p31 was averagely increased 2.9 folds in Cd-pretransformed cells, 4.3 folds in Cd-transformed cells and 7.2 folds in Cd-tumorigenic cells. No change was found n the sequence of overexpressed EF-1beta p31 at different stages of 16HBE cells transformed by CdCl2.</p><p><b>CONCLUSION</b>Overexpression of human EF-1beta p31 is positively correlated with malignant transformation of 16HBE cells induced by CdC12, but is not correlated with DNA mutations.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Régulation de l'expression des gènes tumoraux
/
Lignée cellulaire
/
Transformation cellulaire néoplasique
/
Analyse de séquence d'ADN
/
Chlorure de cadmium
/
Muqueuse respiratoire
/
Facteur-1 d'élongation de la chaîne peptidique
/
Cellules épithéliales
/
Génétique
Limites du sujet:
Humains
langue:
Anglais
Texte intégral:
Biomedical and Environmental Sciences
Année:
2010
Type:
Article
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