Efficient Cultivation Conditions for Human Limbal Epithelial Cells
Journal of Korean Medical Science
;
: 864-869, 2008.
Article
Dans Anglais
| WPRIM
| ID: wpr-37029
ABSTRACT
To compare the stem niche in different culture conditions of limbal epithelial cells, the suspended human limbal epithelial cells (HLECs) were seeded on the 3T3-pretreated plates and the other suspended cells were plated on amniotic membranes (AMs) which were either cryo-preserved or freeze-dried. All were cultured for 10 to 12 days. Reverse transcription-polymerase chain reaction (RT-PCR) for ATP-binding casette, subfamily G, member 2 (ABCG2), p63, cytokeratin 12, and connexin 43 were performed in cultivated HLECs and their expression levels were compared. The mRNA expression of all markers examined showed no statistically significant differences between the cells on cryo-preserved and on freeze-dried AM. The expression of p63 and cytokeratin 12 in cultivated cells on AMs were significantly lower than those in 3T3-cocultured cells on RT-PCR and immunofluorescent staining. Cultivated HLECs on AMs showed reduced proliferation and differentiation while maintaining stem-property regardless of the preservative method of AM.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phosphoprotéines
/
Cellules souches
/
Immunohistochimie
/
Transactivateurs
/
Cellules cultivées
/
Techniques cytologiques
/
Cellules 3T3
/
Amorces ADN
/
Techniques de culture cellulaire
/
RT-PCR
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Humains
langue:
Anglais
Texte intégral:
Journal of Korean Medical Science
Année:
2008
Type:
Article
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