The Effect of Human Placental Extracts in Suppressing Tumor Cell and Preventing Normal Cell Damage / 日本補完代替医療学会誌
Japanese Journal of Complementary and Alternative Medicine
;
: 65-71, 2015.
Article
Dans Japonais
| WPRIM
| ID: wpr-377326
ABSTRACT
Objectives:
To analyze the effect of human placental extracts (HPEx) on hepatocellular carcinoma cells in vitro.Methods:
The hepatocellular carcinoma cell lines, namely, HLE and Huh-7, were used. The cells were subjected to a growth assay using the formazan dye method; the effect of combination treatment with sorafenib and HPEx was also assessed. The preventing normal cell damage effect of HPEx was analyzed by virtual therapy where possible; the experimental protocol was constructed on the basis of pre- and post-sorafenib treatment data. Cytotoxicity was measured by lactate dehydrogenase (LDH) assay.Results:
HPEx caused significant dose-dependent suppression in the growth of HLE and Huh-7 cells. These tumor cells were significantly suppressed by combination treatment with HPEx and sorafenib. In addition, HPEx potentiated sorafenib sensitivity against tumor cells, and significantly prevented sorafenib-induced cytotoxicity in primary cultured rat hepatocytes under all designed experimental conditions. Specifically, pre-treatment with HPEx had a greater effect than post-treatment with HPEx.Conclusion:
HPEx suppresses tumor cell growth, potentiates sorafenib efficacy, and has a preventing normal cell damage effect; this triple functionality of HPEx makes it a useful agent for liver cancer therapy.
Recherche sur Google
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Guide de pratique
langue:
Japonais
Texte intégral:
Japanese Journal of Complementary and Alternative Medicine
Année:
2015
Type:
Article
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