Effects of Tobacco-Specific Carcinogen on Protein Kinase C Isoforms / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
;
: 666-673, 2003.
Article
Dans Coréen
| WPRIM
| ID: wpr-37996
ABSTRACT
BACKGROUND:
Cigarette smoking is the leading cause of the lung cancer. However, mechanism of action underlying the carcinogenesis in the lung still remains to be elucidated. The present study attempted to look into the carcinogenic potential of tobacco-specific nitrosamine, NNK (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone) and the effects of protein kinase C (PKC) isoforms in an immortalized human epithelial cell model. MATERIAL ANDMETHOD:
Immortalized human epithelial cells were exposed with NNK and examined for its carcinogenic potential as measured by saturation density, soft-agar colony formation, and cell aggregation assay. The specific isoform of PKCs involved in the cellular transformation was analysed through western blot with monoclonal antibody and measured separately in cytosolic fraction and membrane fraction.RESULT:
Human epithelial cells exposed with NNK showed prominent carcinogenic potential in saturation density, soft agar colony formation, and cell aggregation assay. PKC isoform analysis results are as follows PKC-alpha showed significant translocation of protein levels from cytosolic fraction to membrane fraction, as analyzed by immunoblot. PKC-epsilon showed a dose-dependent increase of translocation. PKC-lambda was not affected by NNK treatment.CONCLUSION:
The study demonstrated that there was a certain specificity in the patterns of isoform induction following chemical carcinogen exposure. Thus, it is suggested that identification of specific isoform be a clue to find target molecules in the carcinogenesis.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Protein kinases
/
Nicotiana
/
Protéine kinase C
/
Cancérogènes
/
Fumer
/
Agrégation cellulaire
/
Technique de Western
/
Sensibilité et spécificité
/
Isoformes de protéines
/
Cytosol
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Coréen
Texte intégral:
The Korean Journal of Thoracic and Cardiovascular Surgery
Année:
2003
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS