Detecting serum anti-aquaporin-4 antibody in neuromyelitis optica patients by mimic antigen based ELISA / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
; (12): 965-967, 2010.
Article
de Zh
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| ID: wpr-383158
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WPRO
ABSTRACT
Objective To explore mimic antigen based ELISA for detecting serum anti-acquaporin4(AQP4) antibody in neuromyelitis optica(NMO) patients. Methods Three polypeptides: AQP456-69,AQP4135-155, AQP4209-230 were designed to simulate antigen epitopes of AQP4 through biological information and structure biology analysis, the peptides was used as antigen in ELISA to detect serum anti-AQP4 antibody in 9 NMO patients and 7 other miscellaneous neurological disorders which hayed been detected by immumofluorescence method. Results The mean value of A of anti-AQP4 antibody postive patients which have been determined by immumofluorescence method were higher than the controls in ELISA with AQP4135-155,AQP4209-230 as antigen (P<0.05). When the patients serum were diluted at 4 and 8 times, the A values were higher than controls significantly ( P<0.05 ). Conclusion Outmembrane polypeptides AQP4135-155,AQP4209-230 may be the main antigen epitope or main part of antigen epitope, they could be used to detect serum anti-AQP4 antibody in NMO patients.
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WPRIM
langue:
Zh
Texte intégral:
Chinese Journal of Microbiology and Immunology
Année:
2010
Type:
Article