Observation of acute pancreatitis microcirculation with confocal laser endoscopy / 中华消化内镜杂志

ABSTRACT

Objective To study microcirculation of acute pancreatitis(AP)in vivo with confocal la-ser endoscopy(CLE).Methods Thirty-two SD rats were randomly divided into 2 groups，I.e.control(n=8)and experiment group(n=24).In the control group，pancreas were identified，the blood was collectedfrom the inferior vena cava,0.5％uranin was administered at 4 ml/kg intravenously,and the pancreas were observed through CLE.In the experiment group,AP model was established by ligation of the end of common bile duct.The abdomen was opened to observe the pancreas at 12 h(n=8)，24 h(n=8)and 48 h(n=8)， uranin was administered intravenously，and the pancreatic tissue was observed through CLE.Pancreatic tis- sue in each group was sent for pathological study.Results Both healthy and affected pancreatic microcircu- lation can be viewed through CLE.The number of red blood cells can be counted within certain period of time cross-sectionally.In 12 h group，the construction of pancreatic acini was normal，and the flow of red blood cells could be seen in the capillary，but quite slow.In 24 h group，the structure was vague，but there were still normal pancreatic acini less visible capillaries and less and slower red blood cells flow were ob- served.In 48 h group，some pancreatic acini were not visible，normal pancreatic acinar structure and micro- circulation could not be observed，neither could red blood cells.Insulin tissue，white blood cells and micro- thrombosis could not be observed through CLE.ConclusionCLE is distinctly valuable for pancreatic mi- crocirculation study，especially for AP microcirculation in vivo.However，color developing agents and image processing techniques should be improved to ensure better images and better application.