Co-circulation of Two Genotypes of Hepatitis A Virus from Sporadic Cases in Northeastern Area of Seoul, Korea / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 371-377, 2008.
Article
Dans Coréen
| WPRIM
| ID: wpr-39343
ABSTRACT
BACKGROUND:
In previous studies, most hepatitis A virus (HAV) isolates had been genotype IA in Korea. Recently, a small number of different genotypes were reported with an upsurge of acute hepatitis by HAV. We investigated the distribution of HAV genotypes.METHODS:
RNA was extracted from anti-HAV IgM positive sera which were collected from March 2007 to February 2008 at a tertiary care hospital in Northeastern Seoul, Korea. Nested reverse transcription (RT)-PCR and direct sequencing for VP1/P2A region of the HAV were performed.RESULTS:
A total of 699 cases with suspected acute hepatitis were tested for anti-HAV IgM, and positive results were obtained in 56 sera (8.0%), which were collected 2 to 15 days (median, 7 days)after the onset of symptoms. Of the 56 seropositive samples, 52 (92.9%) were positive for HAV RNA, among which 28 isolates (53.8%) belonged to genotype IA and the remaining 24 (46.2%) belonged to genotype IIIA. Both IA and IIIA genotypes were isolated from 6-7 neighboring administrative districts throughout the year without geographic or seasonal restrictions.CONCLUSIONS:
Co-circulation of two distinct HAV genotypes (IA and IIIA) was observed from the northeastern Seoul for the year studied.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Phylogenèse
/
Immunoglobuline M
/
ARN viral
/
Données de séquences moléculaires
/
Protéines virales structurales
/
Séquence d'acides aminés
/
Analyse de séquence d'ADN
/
Virus de l'hépatite A humaine
/
RT-PCR
/
Génotype
Limites du sujet:
Adolescent
/
Adulte
/
Enfant
/
Femelle
/
Humains
/
Mâle
Pays comme sujet:
Asie
langue:
Coréen
Texte intégral:
The Korean Journal of Laboratory Medicine
Année:
2008
Type:
Article
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