Assessment of Hemorheological Deformability of Human Red Cells Exposed to tert-Butyl Hydroperoxide, Verapamil and Ascorbate by Ektacytometer / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
;
: 325-331, 2008.
Article
Dans Coréen
| WPRIM
| ID: wpr-39349
ABSTRACT
BACKGROUND:
Normal erythrocyte is deformable and this facilitates blood flow in the capillaries. Oxidative stress reduces the deformability of erythrocytes, and influences on blood flow in microcirculation. The objective of this study was to investigate the deformability of erythrocytes exposed to oxidative stress, the protective effects of verapamil and ascorbic acid against oxidative damages in erythrocytes, and the value of the microfluidic ektacytometer, RheoScan-D (RheoMeditech, Korea) in clinical application.METHODS:
Effects of oxidative stress on erythrocytes were investigated using tert-butyl hydroperoxide (tBHP). Before exposure to tBHP, the erythrocytes were pretreated with verapamil and ascorbic acid to examine their protective effect against oxidative damages. The deformability of erythrocytes was measured by the microfluidic ektacytometer, RheoScan-D.RESULTS:
When treated with tBHP, the deformability of erythrocytes was decreased (P<0.01) and methemoglobin (metHb) formation and mean corpuscular volume (MCV) of erythrocytes were increased (P<0.01, P<0.05) compared to those of the untreated control cells. Compared to the tBHP treated cells, pretreatment with verapamil increased the deformability of erythrocytes (P<0.01) and decreased metHb formation (P<0.01) and MCV (P<0.05). Likewise, pretreatment with ascorbic acid increased the deformability of erythrocytes (P<0.01) and decreased metHb formation (P<0.01).CONCLUSIONS:
Oxidative stress reduces the deformability of erythrocytes and the deformability could be one of markers for oxidative damage. Verapamil and ascorbic acid have protective role against tBHP induced oxidative stress. The ektacytometer, RheoScan-D used in this study is convenient for clinical measurement and could be used in various fields of clinical medicine.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Acide ascorbique
/
Inhibiteurs des canaux calciques
/
Vérapamil
/
Statistique non paramétrique
/
Stress oxydatif
/
2-Hydroperoxy-2-méthyl-propane
/
Techniques d'analyse microfluidique
/
Déformabilité érythrocytaire
/
Érythrocytes
/
Antioxydants
Limites du sujet:
Adulte
/
Humains
/
Mâle
langue:
Coréen
Texte intégral:
The Korean Journal of Laboratory Medicine
Année:
2008
Type:
Article
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