A study of association between polymorphisms of apolipoprotein M gene and cerebral infarction / 中华神经科杂志

ABSTRACT

Objective To investigate the association between the polymorphisms of apolipoprotein M gene (ApoM) and the risk for cerebral infarction in Han population in northern China.Methods The polymorphisms of ApoM gene were identified by PCR-DNA sequencing analysis and were subsequently detected by PCR-RFLP in 560 patients with cerebral infarction and 550 control subjects in northern China. Statistic analysis was conducted using the SPSS 10.0 program. The linkage disequilibrium (LD) was analyzed by EH and SHEsis softwares. Results Three single nucleotide polymorphisms were found in intron 1 and intron 5 of ApoM gene. The frequencies of GA+AA genotype and A allele of rs805264, GT +TT genotype and T allele of rs707922, CA+AA genotype and A allel of rs707921 were significantly higher in the patients (33.8% and 17.9%, 34.5% and 18.3%, 33.4% and 17.8%, respectively) than in controls (21.1% and 11.2%, 21.8 % and 11.5%, 20.9% and 11.1%, respectively). The LD was found in rs805264, rs707922 and rs707921 of ApoM gene (χ2=2595.03, P<0.01). There was strong LD between each pair of the three markers (D′=0.972 to 0.992). Multiple logistic regression analysis revealed that the A-T-A haplotype of the ApoM gene was an independent risk factor for cerebral infarction (OR=1.780;95%CI=1.333-2.376, P<0.01). Conclusions GA genotype and A allele of rs805264, GT genotype and T allele of rs707922, CA genotype and A allele of rs707921 may be genetic risk factors for cerebral infarction. A-T-A haplotype of ApoM gene may be a susceptible genotype of cerebral infarction.